Yeah, sure.
You don’t have to change any thing from the hosted zone.
All you have to do is go to Route 53 -> Registered Domains -> Find the domain you want to configure -> Manage DNS (As shown in the image) and then, all to the right you have an option called “Add or edit name servers”. Click ther and replace all the values with your Netlify DNS values and then wait a little bit until the changes are applied